SIV mac251直肠途径感染猕猴模型

1.该模型制备比较成熟，国内外都有此模型，构建和评价指标一致，所以该模型应用广泛，具有统一的操作规程。

2. 该模型制备的关键：1）小鼠必须是重度免疫缺陷的，而且饲养环境必须洁净；2）HIV操作需要生物安全。

3.此模型具有完善的人的免疫系统，HIV感染后，具有艾滋病病人相似的临床症状，所以具有很高的比较医学应用价值。

所有感染动物必须在ABSL-3室中进行。

诊断：病毒在血液中复制。

Three vaccines were tested in groups of 8 MCM with each animal being vaccinated intradermally or intramuscularly with 100μg DNA primes at weeks 0, 4 and 8 and a rAd5 boost (6x1010 particles) at week 16.Eight unvaccinated animals served as controls for each group challenged with the same virus stock.The IM vaccination experiments were performed subsequent to the ID vaccinations and employed an additional group of 8 control animals that were challenged in parallel. From week 23 the animals were challenged intrarectally with 150TCID50 of SIVmac251 at weekly intervals for up to 10 weeks or until they became infected, as determined by virus in the plasma.

来源文献：Vaccination of macaques with DNA followed by adenoviral vectors encoding SIV gag alone delays infection by repeated mucosal challenge with SIV.

PMID：31413132

DOI：10.1128/JVI.00606-19